Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Canine vascular endothelial cells plated on a fibrin matrix exposed to treatment media for 5 days. A: Cells treated with Mesencult. B: Cells treated with Mesencult containing insulin, transferrin and sodium selenite. C: Cells treated with conditioned media.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques:

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: A: Cells stained with acridine orange on the underside of the 3 µm polycarbonate membrane after MIP-1α treatment. Yellow-green = DNA; red = RNA. B: Effect of VEGF, MCP-1 and MIP-1α on MSC migration. Data expressed as a mean percent of Mesencult (control) treated cultures ± SE (n = 6). a = p<0.05 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Staining, Membrane, Migration, Control

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes) or conditioned media (CM) for 24 hours under hypoxic conditions. Data calculated as a mean percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Control

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), MCP-1 or MCP-1 + PI 3-Kγ inhibitor for 24 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Control

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: H9c2 cells treated for 24 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Control

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: H9c2 cells treated for 6 or 24 hours under hypoxic conditions. Data calculated as a percent of the 6-hr Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Control

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Secrete Multiple Cytokines That Promote Angiogenesis and Have Contrasting Effects on Chemotaxis and Apoptosis

doi: 10.1371/journal.pone.0035685

Figure Lengend Snippet: Changes in phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), conditioned media (CM) or ERK 1/2 inhibitor for 6 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.

Article Snippet: Briefly, bone marrow cells were enriched for lineage negative (Lin−) cells using the SpinSep system (Stem Cell Technologies) and plated on tissue culture treated plates at a density of 0.1×10 6 cells/cm 2 in murine Mesencult media (basal media + stimulatory supplement; Stem Cell Technologies) with 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B added.

Techniques: Control